At3g53460.2
From Proteins Wiki
In the plant Arabidopsis thaliana, At3g53460.2 is a gene model for the locus At3g53460.[1]
Also known as CP29, this gene model is classified by TAIR as "CP29 (chloroplast 29 kDa ribonucleoprotein); RNA binding". It is located on chr3, region 19830716-19832401 REVERSE.
Contents |
[edit] Physiological function
At3g53460.2/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
[edit] Tissue expression
[edit] Subcellular localization
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Biophysical properties
The gene model At3g53460.2 has 334 amino-acid residues and a molecular weight Mw = 35.237 kDa.
The protein is relatively acidic; at the physiological pH 7.4, its net charge is -6.00, or -0.018 per residue. NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Knockout phenotype
[edit] Protein interactions
At3g53460.2/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
[edit] Domain structure
The gene model At3g53460.2 has 2 recognized domains:
- Domain_0101_0172 RRM_1 domain (PF00076, IPR000504) Description: RNA-binding region RNP-1 (RNA recognition motif);Molecular Function: nucleic acid binding (GO:0003676)
- Domain_0251_0322 RRM_1 domain (PF00076, IPR000504) Description: RNA-binding region RNP-1 (RNA recognition motif);Molecular Function: nucleic acid binding (GO:0003676)
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
[edit] Amino-acid sequence
>At3g53460.2
MSASASSLSA
FNPKSLPLCV
SRPASVSVLP
PSLSFKLHSD
HLVSIFASSA
LKCSSPAEYP
SRFVRNVAVS
SDFEVEEDDM
FADGDDSAPV
ERNSFSPDLK
LFVGNLSFNV
DSAQLAQLFE
SAGNVEMVEV
IYDKVTGRSR
GFGFVTMSTA
AEVEAAAQQF
NGYEFEGRPL
RVNAGPPPPK
REESFSRGPR
SGGYGSERGG
GYGSERGGGY
GSERGGGYGS
QRSGGGYGGS
QRSSYGSGSG
SGSGSGSGNR
LYVGNLSWGV
DDMALENLFN
EQGKVVEARV
IYDRDSGRSK
GFGFVTLSSS
QEVQKAINSL
NGADLDGRQI
RVSEAEARPP
RGQF
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
[edit] Multiple sequence alignment
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
[edit] Protein structure
A predicted structure for this protein is available here.
[edit] References
- ↑ This is a test reference.
